Role of CCL2/CCR2 axis in the increased mobilization of inflammatory monocytes in D6^−/− mice spleen. (A) CCL2 concentrations in the serum of D6^−/− (black bar) and WT (white bar) mice 4 hours after intravenous injection of 2 μg hCCL2. Data are mean ± SD of 2 experiments with 4 mice/group. (B) Fold increase of the absolute number of CD11b⁺/Ly6C^high monocytes recovered from spleen of D6^−/− (■) versus WT (□) mice at indicated time points after injection (n = 8 mice/time point). *P < .05, D6^−/− versus WT mice. (C-D) Fold increase of the absolute number of CD11b⁺/Ly6G⁺ neutrophils (C) and CD11b⁺/Ly6C^high monocytes (D) in spleen of D6^−/− mice reconstituted with WT or D6^−/− or CCR2^−/− BM cells versus WT mice reconstituted with WT BM cells. Spleens were analyzed 2 days after subcutaneous injection of CFA (200 μL). Data are mean ± SD (n = 8 mice/group). *P < .05. **P < .005. ***P < .0005.