Platelet function studies. (A) Aggregation of human PRP was done to compare the effect of PRT318 on convulxin versus ADP. Aggregation of PRP treated with PRT318 (0-50μM) was determined in response to convulxin (8 ng/mL) or ADP (5μM). (B) Human platelet calcium flux was measured in a 96-well format assay to compare the effects of PRT318 on convulxin versus the PAR1 agonist, TRAP-6 (SFLLRN). Calcium flux in platelets treated with PRT318 (0-16μM) was measured in response to convulxin (125 ng/mL) or TRAP-6 (3μM).