Identification of miRs enriched in CD34+ EB cells. (A) miRs up-regulated and enriched in CD34+ cells derived from H1 EB differentiation. Levels of miRNA expression were determined by TaqMan real-time PCR analysis. Values of fold change in expression are relative to day 7 CD34− EB cells. (B) Kinetic analysis of pluripotency, mesodermal, hematopoietic genes, and miRs in differentiating EB cells. EBs were harvested on the indicated days, and levels of gene expression were determined by quantitative real-time PCR analysis. Values of fold change in expression are relative to undifferentiated H1 hESCs.