Generation of inducible miRs-126/126* overexpressing hESCs. (A) Schematic representation of pLTET1 and pLTRET1-miR lentiviral vectors used to derive inducible miR expressing hESCs (magnification 100×). Phase contrast and fluorescent micrographs of live cells in tissue culture media were acquired using a Zeiss Axiovert 200 model microscope with ECPlan Neofluor 10×/0.3 Ph1 objectives. Image was captured using an Optronics MagnaFIRE camera with MicroFIRE Version 070121-00X1 software. (B) Phase-contrast and immunofluorescence microscopy images of I-miRs-126/126* hESCs. (C) A total of 1 μg/mL Dox treatment induced miR-126 and 126* expression. miR expression is presented as fold induction relative to expression in day 20 EB cells.