Vascular Nogo-A/B regulates neutrophil infiltration into air pouches. Nogo-A/B^−/− mice displayed a significant reduction of (A) carrageenan and (B) zymosan (1% or each, wt/vol)-induced neutrophil recruitment into the air pouches 4 hours after instillation compared with WT mice (n = 5 per group). (C) Four hours after intrapouch injection of carrageenan, leukocytes were recovered and evaluated for lineage molecule expression Gr-1 (neutrophil marker) and CD68 (monocyte/macrophage marker) on leukocytes isolated from the pouch or (D) 24 hours for evaluation of protein expression by Western blot analysis (n = 4 per group). (E) Carrageenan and (F) zymosan air-pouch models were created in chimeric mice WT → Nogo-A/B^−/−, Nogo-A/B^−/− → WT and control WT → WT, Nogo-A/B^−/− → Nogo-A/B^−/− (n = 5 per group). Mice were killed 4 hours after instillation, and viable cells recovered from the pouches were counted using trypan blue. The data shown represent the means ± SEM. *P < .05; **P < .01 compared with WT group; ^#P < .05 compared with Nogo-A/B^−/− → WT group.