The absence of Nogo-B does not influence fMLP- or IL-8–induced bone marrow–derived neutrophil migration or BMDM activation in vitro. (A) Bone marrow–derived neutrophils were isolated from the bone marrow of 8- to 12-week-old mice. Western blot analysis for Nogo-B in bone marrow–derived neutrophils is shown. (B) Representative histograms of adhesion and lineage molecules (Gr-1, CD11b, CD18, CD11a, and P-selectin glycoprotein ligand-1 [PSGL-1]) in WT (black line) and Nogo-A/B^−/− (red line) bone marrow–derived neutrophils with isotype-matched control labeling (gray dotted line). Chemotaxis toward (C) fMLP (0.1, 0.3, and 1 μM) and (D) IL-8 (1, 30, and 50 nM) was performed in bone marrow–derived neutrophils from WT and Nogo-A/B^−/− mice, as described in “Methods.” (E) Adhesion and lineage molecules in WT (black line) and Nogo-A/B^−/− (red line) BMDMs and isotype-matched control antibody (gray dotted line). (F) Relative expression of cytokines by quantitative PCR in exudate-stimulated WT and Nogo-A/B^−/− BMDMs. (G) Western blot analysis of WT and Nogo-A/B^−/− BMDMs stimulated for 24 hours with exudates. The data shown represent the means ± SEM from 3 individual experiments.