Characterization of genome-wide GABPα binding locations in human HPCs. (A) GABP binding at selected gene loci inferred from ChIP-Seq. Chromatin fragments prepared from CD34⁺CD133⁻ human HPCs were subjected to ChIP-Seq analysis. For each gene locus (in individual panels), sequence tags from anti-GABPα (first row) and control IgG (second row) samples were displayed on the University of California Santa Cruz genome browser with their heights (y-axis) denoting the tag numbers. GABP binding locations identified by SISSRs, highlighted in red rectangles, are displayed as enrichment peaks in the third row of each panel, with the y-axis heights corresponding to fold-enrichment of GABP sequence tags over control tags. Gene symbols and corresponding protein names (in parentheses), scale, and relative locations in respective chromosomes are shown on the top, and gene structures and directions of transcription are marked at the bottom of each panel. (B) Genome-wide distribution of GABPα binding locations in human HPCs. TIS indicates transcription initiation site; UTR, untranslated region. (C) Correlation between GABP binding and gene expression in human HPCs. Genes sorted by absolute expression levels in human HPCs (from Cui et al²⁶ ) were binned into 10 groups, and the percentages of genes within each group that bind GABP within 2 kb of TISs are shown.