Analysis of CCR7 uptake by NK cells. (A) A representative flow cytometric analysis of CD56, CD16, and CCR7 expression on buffy coat–derived CD3⁻CD56⁺ NK cells before and after expansion. (B) Flow cytometric analysis of CCR7 acquisition by NK cells after 30 minutes of coculture with Clone9.CCR7 cells compared with Clone9.mbIL21 cells. NK cells were initially discriminated from the K562 cells on the basis of the forward (FSC-H) and side (SSC-H) scatter properties and further gated on CD56⁺ NK cells to ensure analysis of CCR7 in NK cell population. (C) Kinetics of CCR7 uptake by NK cells from irradiated Clone9.CCR7 cells as analyzed with flow cytometry. The uptake of CCR7 was analyzed hourly for ≤ 8 hours compared with NK cells cocultured with Clone9.mbIL21. The data are presented as the median ± range for 3 unrelated NK cell donors.