Correlation of uptake of multiple receptors by NK cells with expression of the receptors on donor cells. (A) Flow cytometric analysis of NK cells after 30 minutes of coculture with parental K562 (black histogram) and clone9.CCR7 (unfilled histogram) cells. NK cells acquired CCR7, mbIL21, CD137L, CD64, truncated CD19, and CD86 transgenes that were expressed on Clone9.CCR7 cells.(B) Transgene expression on parental K562 (black histogram) and Clone9.CCR7 (unfilled histogram) cells. (C) Nonlinear regression (curve fit) analysis of the correlation between the mean fluorescence intensity (MFI) of acquired receptors on NK cells and their corresponding MFI on donor cells. The correlation as calculated by Pearson test was statistically significant (P < .0034). (D) Expression of CD16 and CD56 in NK cells before (black histogram) and after (unfilled solid histogram) coculture with parental K562 or Clone9.CCR7 cells (unfilled dotted histogram). (E) Kinetics of loss of CD64, CD86, and CD137L from NK cells derived from 3 different persons (n = 3). The data are represented as median ± range.