Ablation of A20 in the B-lineage has B cell-extrinsic effects on immune homeostasis. (A) Dot-plots showing percentages of CD4⁺Foxp3⁺ regulatory T cells and CD4⁺CD25⁻ T cells (naïve indicates CD44^intCD62L^hi; memory, CD44^hiCD62L^hi; and effector, CD44^hiCD62L^lo) in the spleen. Numbers indicate the mean of 4 to 6 mice for each genotype. (B-D) Absolute cell numbers of CD4 T (B), CD8 T (C), and myeloid cell (D) subsets in B^A20−/−, B^A20+/−, and CD19cre mice (n = 6 per group; 8-12 weeks old). Data are mean ± SD. Treg indicates Foxp3⁺; naive, CD44^intCD62L^hi; memory, CD44^hiCD62L^hi; effector/memory, CD44^hiCD62L^lo; DC, dendritic cell (CD11c⁺); Eos, eosinophils (Gr1^intSiglecF⁺); Mac, macrophages (Mac1⁺Gr1^lo); and Neu, neutrophils (Gr1^hiLy6G⁺). (E) Intracellular cytokine staining of ex vivo isolated splenocytes (gated on T cells). Numbers represent mean plus or minus SD of 3 mice per genotype. *P < .05 (1-way analysis of variance). **P < .001 (1-way analysis of variance).