A20 controls canonical NF-κB activation in response to B-cell mitogens. (A-C) Western blot of whole cell lysates stimulated for the indicated time points with (A) 10 μg/mL αIgM, (B) 20 μg/mL LPS, and (C) 10 μg/mL αCD40. (D) Western blots on cytoplasmic and nuclear extracts after stimulation with αCD40. Results are representative of 2 or 3 independent experiments.