Isolation and analysis of a NOTCH1 intragenic deletion in human T-ALL. (A) Chromosome 9 ideogram showing deletions identified by aCGH encompassing the CDKN2A/B tumor suppressor gene and the NOTCH1 locus in T-ALL. (B) aCGH plot showing an intragenic focal deletion of NOTCH1 in chromosome band 9q34. (C) Agarose gel electrophoresis of a truncated NOTCH1 cDNA sequence isolated by 5′-RACE using a primer located in exon 28 of NOTCH1. (D) Nucleotide sequence of the NOTCH1 5′ RACE product. (E) Kozak consensus sequence alignment of the NOTCH1 sequences surrounding methionine 1668 located at the N-terminal portion of the NOTCH1 del-N amino acid sequence. R indicates purine (adenosine or guanosine).