GFP(PAX5)⁺B220⁺GR-1/MAC-1⁺ cells coexpress genes characteristic for both lymphoid and myeloid cells. (A) Diagrams of TaqMan Q-RT-PCR analysis of gene expression in 2 pre-B cell lines 230-238 (B1) and 18-81 (B2), WEHI3 cell line (W), and 3 GFP(PAX5)⁺B220⁺GR-1/MAC-1⁺ long-term cell lines (Exp no. 1, 190 days; Exp no. 2, 80 days; Exp no. 3, 65 days) independently generated through PAX5 transduction of LSK cells. (B) GFP⁺ cells were sorted from control (□) and PAX5-transduced (▪) cultures at indicated times and RNA extracted for gene expression analysis. Differences in cDNA input were compensated by normalizing against Hprt expression levels. Mean (SD) values of 3 experiments. *P < .05. All water controls were negative (data not shown).