Analysis of differentiation, activation, and migration markers on CD4⁺ and CD8⁺ T lymphocytes expressing CCR6. Differentiation markers CD27, CD45RA, CD28, CD127 (A-D), activation markers CD95, HLA-DR, CD38, and CD57 (B,E), and migration markers such as CCR7, CD62L, CCR5, and CXCR3 (C,F) were studied for their expression by CD4⁺ T cells (A-C) and CD8⁺ T cells (D-F). For flow cytometric analysis, 15 000 events were accumulated. Representative staining of PMBCs from 8 UI individuals is shown. Percentages of cells in each quadrant are indicated. Because antibody directed against CD62-L was conjugated to FITC fluorochrome, the CCR6 antibody used in the figure was conjugated to PE unlike all others stainings that used CCR6-FITC.