CCL20 released by splenocytes and surface expression of CCR6 on CD4⁺ splenocytes: comparison of uninfected and HIV-infected individuals. (A) CCL20 released by splenocytes at different times during culture was determined by ELISA and is expressed in picograms per milliliter for uninfected (dotted line) and HIV-infected (solid line) individuals. (B) Surface expression of CCR6 was determined by flow cytometry in CD4⁺ splenocytes isolated from the spleen of healthy individuals (□) and HIV-infected patients (▪) and presented as the percentage of CCR6⁺ splenocytes within CD4⁺. *P < .05 **P < .01 ***P < .001. Error bars represent SEM.