American Society of Hematology

Figure 1

$Figure 1. Expression of Gli1 and Gli2\#916;N2 in WT and Gli2\#916;N2 transgenic mice. (A) Hh activation status, shown by quantitative RT-PCR analysis of gli1 mRNA in FACS-sorted populations of the adult murine thymus. (B) Quantitative PCR of genomic Gli2\#916;N2, showing high copy number of the Gli2\#916;N2 transgene. (C) Relative Gli2\#916;N2 expression, by quantitative RT-PCR analysis, in thymus, lymph node, and spleen (D) and up-regulation of Gli1 transcription in Gli2\#916;N2 transgenic CD4SP thymocytes. Error bars show standard deviation (SD). Data are representative of at least 3 experiments.$

Expression of Gli1 and Gli2\#916;N₂ in WT and Gli2\#916;N₂ transgenic mice. (A) Hh activation status, shown by quantitative RT-PCR analysis of gli1 mRNA in FACS-sorted populations of the adult murine thymus. (B) Quantitative PCR of genomic Gli2\#916;N₂, showing high copy number of the Gli2\#916;N₂ transgene. (C) Relative Gli2\#916;N₂ expression, by quantitative RT-PCR analysis, in thymus, lymph node, and spleen (D) and up-regulation of Gli1 transcription in Gli2\#916;N₂ transgenic CD4SP thymocytes. Error bars show standard deviation (SD). Data are representative of at least 3 experiments.

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