Phenotype of Gli2\#916;N₂ transgenic mice. (A) Relative cell number (calculated relative to the mean of WT littermates) in the thymus and spleen of WT (gray bars, n = 6) and Gli2\#916;N₂ transgenic (\#9633;, n = 6). (B) CD4 and CD8 profiles in the Gli2\#916;N₂ transgenic mouse. Thymocytes from Gli2\#916;N₂ transgenic and WT littermates were analyzed for CD4 and CD8 expression in the thymus (top panels), spleen (middle panels), and lymph node (bottom panels). Differences between the percentage of populations between transgenic and littermate were statistically significant as given: (thymus) CD4SP, P = .005; DP, P = .014; (spleen) CD4, P = .002; CD8, P = .001; and (lymph node) CD4, P = .005; CD8, P = .041. (C) Bar chart to show change in CD4/CD8 ratio in thymus (WT [n = 6], Gli2\#916;N₂ [n = 6], P = .032). (D) CD3 expression on CD4 (top histograms) and CD8 (bottom histograms) SP thymocytes. (E) HSA expression on CD4 (top) and CD8 (bottom) SP thymocytes. (F) CD2 expression on DP (top), CD4SP (middle), and CD8SP (bottom) cells in the Gli2\#916;N₂ transgenic (right panels) and the WT (left panels). (G) CD5 expression on CD8SP cells in the Gli2\#916;N₂ transgenic thymus (gray line) compared with the WT (black line). The bar chart shows mean MFI for WT (\#9641;) and Gli2\#916;N₂ transgenic (\#9633;). (H) CD69 expression on DP (top), CD4 (middle), and CD8 (bottom) SP thymocytes. Numbers in dot plots or histograms indicate the percentage of cells falling in the quadrant, region, or marker. Error bars show standard error (SE). Data are representative of at least 3 experiments.