American Society of Hematology

Figure 5

$Figure 5. Gli2\#916;N2 expression influences TCR repertoire selection. (A) Thymus phenotype of male mice with the transgenic HY-TCR. CD4 and CD8 expression in HY-TCR\#8211;positive mice with (Gli2\#916;N2HY-TCR) and HY-TCR littermates without the Gli2\#916;N2 transgene. Mean thymus sizes were 2.4 × 107 for HY-TCR and 2.3 × 107 for Gli2\#916;N2HY-TCR. The histograms show DbSmcy tetramer, V\#946;8, and CD69 staining on CD8SP cells from the Gli2\#916;N2HY-TCR. The bar chart shows the percentage of CD8 DbSmcy-positive cells in the HY-TCR (n = 12) and Gli2\#916;N2HY-TCR (n = 8) thymus (P = < .001). (B) Peripheral phenotype of male mice with the HY transgenic TCR. CD4 and CD8 profile of a Gli2\#916;N2HY-TCR lymph node. The bar chart shows the percentage of CD8 DbSmcy-positive cells in HY-TCR (n = 12) and Gli2\#916;N2HY-TCR (n = 8) lymph node (P = < .001). The histograms show DbSmcy and V\#946;8 expression on CD8 T cells from the Gli2\#916;N2HY-TCR. (C) Lymph node of male mice with the B6.2.16\#946; transgenic TCR. Bar chart to show the relative number of CD8+ DbSmcybright cells in B6.2.16\#946;-positive mice (B6) and B6.2.16\#946;Gli2\#916;N2-positive mice (Gli2\#916;N2B6) (P = .021, n = 4, n = 5, respectively). The histograms show V\#946;8 expression on CD8 T cells. (D) Thymus phenotype of HY-TCR female mice. CD4 and CD8 profiles of HY-TCR and Gli2\#916;N2HY-TCR mice (left dot plots). Mean thymus sizes were 8.7 × 107 for HY-TCR and 8.4 × 107 for Gli2\#916;N2HY-TCR. CD4 and CD8 profiles gated on DbSmcy-positive cells in the HY-TCR and Gli2\#916;N2HY-TCR thymus (right dot plots). The percentages of cells falling in the DbSmcy-positive gate were typically 9.6% in the HY-TCR and 9.4% in the Gli2\#916;N2HY-TCR. (E) Peripheral phenotype of HY-TCR female mice. CD4 and CD8 profiles in HY-TCR and Gli2\#916;N2HY-TCR mice. Numbers in dot plots or histograms indicate the percentage of cells falling in the quadrant, region, or marker. Error bars show SE. Data are representative of at least 3 experiments.$

Gli2\#916;N₂ expression influences TCR repertoire selection. (A) Thymus phenotype of male mice with the transgenic HY-TCR. CD4 and CD8 expression in HY-TCR\#8211;positive mice with (Gli2\#916;N₂HY-TCR) and HY-TCR littermates without the Gli2\#916;N₂ transgene. Mean thymus sizes were 2.4 × 10⁷ for HY-TCR and 2.3 × 10⁷ for Gli2\#916;N₂HY-TCR. The histograms show D^bSmcy tetramer, V\#946;8, and CD69 staining on CD8SP cells from the Gli2\#916;N₂HY-TCR. The bar chart shows the percentage of CD8 D^bSmcy-positive cells in the HY-TCR (n = 12) and Gli2\#916;N₂HY-TCR (n = 8) thymus (P = < .001). (B) Peripheral phenotype of male mice with the HY transgenic TCR. CD4 and CD8 profile of a Gli2\#916;N₂HY-TCR lymph node. The bar chart shows the percentage of CD8 D^bSmcy-positive cells in HY-TCR (n = 12) and Gli2\#916;N₂HY-TCR (n = 8) lymph node (P = < .001). The histograms show D^bSmcy and V\#946;8 expression on CD8 T cells from the Gli2\#916;N₂HY-TCR. (C) Lymph node of male mice with the B6.2.16\#946; transgenic TCR. Bar chart to show the relative number of CD8⁺ D^bSmcy^bright cells in B6.2.16\#946;-positive mice (B6) and B6.2.16\#946;Gli2\#916;N₂-positive mice (Gli2\#916;N₂B6) (P = .021, n = 4, n = 5, respectively). The histograms show V\#946;8 expression on CD8 T cells. (D) Thymus phenotype of HY-TCR female mice. CD4 and CD8 profiles of HY-TCR and Gli2\#916;N₂HY-TCR mice (left dot plots). Mean thymus sizes were 8.7 × 10⁷ for HY-TCR and 8.4 × 10⁷ for Gli2\#916;N₂HY-TCR. CD4 and CD8 profiles gated on D^bSmcy-positive cells in the HY-TCR and Gli2\#916;N₂HY-TCR thymus (right dot plots). The percentages of cells falling in the D^bSmcy-positive gate were typically 9.6% in the HY-TCR and 9.4% in the Gli2\#916;N₂HY-TCR. (E) Peripheral phenotype of HY-TCR female mice. CD4 and CD8 profiles in HY-TCR and Gli2\#916;N₂HY-TCR mice. Numbers in dot plots or histograms indicate the percentage of cells falling in the quadrant, region, or marker. Error bars show SE. Data are representative of at least 3 experiments.

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