![Figure 5. Alternatively activated SP37A3 cells exhibit tolerogenic potential. (A) SP37A3 cells were differentiated as described (Figures 1,3). Irradiated cells (2.5 × 105) were cocultured for 7 days with 106 nylon wool–enriched allogeneic BALB/c T cells in 1 mL culture medium. Culture supernatants were harvested and assayed by ELISA for contents of IFN-γ, IL-4, and IL-10. Data represent mean ± SEM of at least 3 experiments. *P < .05. (B-D) Differentially conditioned (Figures 1,3) irradiated SP37A3 cells (106) were cocultured with 6 × 106 nylon wool–enriched T cells of BALB/c mice in 4 mL volume in wells of 6-well cluster plates for 7 days, and T cells were harvested afterward. (B) T cells (105) primed by immature, mature, or alternatively activated SP37A3 cells were restimulated with irradiated C57BL/6 spleen cells (105) in 0.2 mL culture medium for 4 days. (C-D) Graded numbers of T cells primed by immature, mature, or alternatively activated SP37A3 cells (pTCs) were cocultured with 3 × 105 naive BALB/c T cells (nTCs) and 3 × 105 C57BL/6 spleen cells in 0.2 mL volume for 6 days in triplicate cultures. To account for the doubled number of T cells used in suppression assays (pTC + nTC), T-cell proliferation of 6 × 105 naive T cells (2 × nTC) was determined. T-cell proliferation was assessed as uptake of [3H] thymidine for the final 16 hours of culture. Data represent mean ± SEM of triplicate cultures and are representative of 2 (B) and 3 (C) independent experiments. (D) Assessment of suppressive activity of T cells primed by alternatively activated SP37A3 cells (pTC; 3 × 105). The respective proliferation of naive T cells (nTC; 3 × 105) induced by C57BL/6 spleen cells was arbitrarily set to 1. Data repesent mean ± SEM of 3 independent experiments. *P < .05; **P < .001; ***P < .001 compared with 3 × 105 nTCs (C) or between indicated groups (B,D).](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/109/9/10.1182_blood-2006-07-035576/4/zh80090700400005.jpeg?Expires=1735843529&Signature=SUi7pfUrEWWCJwkFN2PA0FRupjZnwiXq0exDdeRy-0p~aN-Hq64JE0QKQ8SDXXu65~NXjh1JOPutQ3ZfCJEzcCvUMpX7znx8eV59ybAjjX9mxIG3TI~psy~CFlz7qd4A3hpq6qtWbVHplk45IWZPo8u7fhCmMEi8iVtlJH6UtH2AECHzQz4jdR39iEWPYZn6GVUCtiUu4MWzF4gGA-i-xnzg1pTpmsp0HGPqSzN1pCkaj0jFzSx9vWoWJJJQLSadny8VQ4-JBnYZHiXqrUY7AoDzs~TLrdnpCZ0PjtO~qe0xMpuVr9M0tewGuxRvqXFlR2t9HN3T9K-6ckNFdfZMdQ__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Alternatively activated SP37A3 cells exhibit tolerogenic potential. (A) SP37A3 cells were differentiated as described (Figures 1,3). Irradiated cells (2.5 × 105) were cocultured for 7 days with 106 nylon wool–enriched allogeneic BALB/c T cells in 1 mL culture medium. Culture supernatants were harvested and assayed by ELISA for contents of IFN-γ, IL-4, and IL-10. Data represent mean ± SEM of at least 3 experiments. *P < .05. (B-D) Differentially conditioned (Figures 1,3) irradiated SP37A3 cells (106) were cocultured with 6 × 106 nylon wool–enriched T cells of BALB/c mice in 4 mL volume in wells of 6-well cluster plates for 7 days, and T cells were harvested afterward. (B) T cells (105) primed by immature, mature, or alternatively activated SP37A3 cells were restimulated with irradiated C57BL/6 spleen cells (105) in 0.2 mL culture medium for 4 days. (C-D) Graded numbers of T cells primed by immature, mature, or alternatively activated SP37A3 cells (pTCs) were cocultured with 3 × 105 naive BALB/c T cells (nTCs) and 3 × 105 C57BL/6 spleen cells in 0.2 mL volume for 6 days in triplicate cultures. To account for the doubled number of T cells used in suppression assays (pTC + nTC), T-cell proliferation of 6 × 105 naive T cells (2 × nTC) was determined. T-cell proliferation was assessed as uptake of [3H] thymidine for the final 16 hours of culture. Data represent mean ± SEM of triplicate cultures and are representative of 2 (B) and 3 (C) independent experiments. (D) Assessment of suppressive activity of T cells primed by alternatively activated SP37A3 cells (pTC; 3 × 105). The respective proliferation of naive T cells (nTC; 3 × 105) induced by C57BL/6 spleen cells was arbitrarily set to 1. Data repesent mean ± SEM of 3 independent experiments. *P < .05; **P < .001; ***P < .001 compared with 3 × 105 nTCs (C) or between indicated groups (B,D).
