Effects of the PI3 kinase inhibitor LY294002 on pERK, pSTAT5, and pS6 activation. WT and Mx1-Cre, Kras^G12D c-kit⁺ lin^−/low cells that were incubated for 30 minutes in the presence of LY294002 (LY) were stimulated with GM-CSF (10 ng/mL) or SCF (100 ng/mL) for 10 minutes and analyzed by multi-parameter FACS. Identical results were obtained in 2 independent experiments.