Antigen specificity of the generated CMV-CTL line. A CTL line specific for the HLA-A2 binding pp65-CMV antigen–derived peptide was generated from a CMV-positive healthy donor (HLA-A2⁺). The specificity of the CTLs was analyzed in IFN-γ ELIspot assays. The CTL line raised against an HLA-A2–binding pp65-derived peptide, specifically recognized autologous DCs loaded with the corresponding peptide but not an irrelevant HIV-derived peptide. The data shown are representative of 5 independent experiments.