Exogenous loading of DCs with an HLA-A2–specific peptide results in equal stimulation of CTLs. TLR-stimulated DCs were exogenously pulsed with a pp65-derived (black columns) or HIV-derived (gray columns) HLA-A2–binding peptide, respectively, and were used as stimulators in IFN-γ ELIspot assays. Autologous HLA-A2–restricted pp65-peptide–specific CTLs were used as effectors. The data shown are representative of 5 independent experiments.