Treatment with 2ME2 results in DNA damage and cleavage of caspase-3, the extent of which is indirectly proportional to SOD2 expression. (A) DNA damage following treatment with 2ME2 was determined by comet assay. Four representative nuclei are shown. (B) Quantitation of the tail moments from the comet assay. The fold change in mean tail moments from 2ME-treated versus untreated cells is shown. For each treatment, 50 tails were measured. Error bars depict standard deviation. (C) DNA damage in cell lines with altered SOD2 was determined by comet assay. Four representative nuclei are shown. (D) Quantitation of the tail moments of the comet assay. A total of 50 tails were measured, and the mean tail moment is shown. Error bars depict standard deviation. (E) Western blot for cleaved caspase-3 in myeloma cell lines following treatment with 2ME2. Actin serves as the loading control. (F) Western blot for cleaved caspase-3 in myeloma cell lines with altered SOD2 following treatment with 2ME2. Actin serves as the loading control.