RPAs using cell lines with manipulated SOD2 levels. The levels of phosphorylated JAK2 and STAT5 were determined using RPAs and cell lines with manipulated SOD2 expression. (A) Results of 3 arrays for the detection of phosphorylated JAK2, total JAK2, and colloidal gold staining for total protein are shown. (B) Quantitation of JAK2 and STAT5 phosphorylation. The values obtained using linear regression for phosphorylated JAK2 and STAT5 were normalized to total protein printed, which was then normalized to total JAK2 and STAT5 expression, respectively. The fold change in phosphorylated values for SOD2-manipulated lines versus the corresponding control is presented.