NRASQ61K sensitizes for treatment with MEK inhibitors. (A) Hut78 cells were left untreated or treated with 1μM AZD6244, 1μM PD0325901, and 1μM U0126 for 4 hours. Then, cells were lysed, and the basal phosphorylation level of ERK and MEK was assessed by Western blot with specific anti-phospho-ERK and with specific anti-phospho-MEK antibodies. Equal loading was verified by antitubulin antibodies. (B-D) CTCL cell lines were incubated with indicated concentrations of MEK inhibitors U0126 (B), AZD6244 (C), and PD0325901 (D) for 72 hours. Cell growth was measured by Cell Titer Glo according to the manufacturer's instructions. The 50% inhibitory concentration values, at which 50% of the cell growth inhibition compared with dimethyl sulfoxide was observed, were calculated by GraphPad Prism Version 5 software. Data are representative for 3 independent experiments.
