AEtr cells have reduced mitotic index in nocodazole. (A) K562 cells stably expressing AEtr or vector control cells were treated with nocodazole for 18 hours. Propidium iodide (PI)–stained cells were detected by flow cytometry. Representative PI histogram of vector control or AEtr cells following nocodazole treatment for 18 hours. (B) Graph indicates the percentages of vector control and AEtr cells that contained either 2N or 8N DNA after nocodazole treatment as determined by PI staining. The averages for 3 to 5 independent experiments with independent pools are indicated. The standard deviation is indicated above each bar. (C) Mitotic index of control or AEtr cells following nocodazole addition. Phospho–histone H3 staining was performed 0, 4, 8, 14, or 18 hours after nocodazole addition. The error bars in the 18-hour time point indicate the average of 3 independent experiments. (D) The graph indicates the percentage of cells in G0/G1 phase of the cell cycle determined by ModFiT analysis of histograms of PI-stained cells following nocodazole treatment for 0, 8, or 12 hours. (E) K562 vector or AEtr-expressing cells were treated with nocodazole for 18 hours followed by addition of 10 μM BrdU for 24 hours. Cells were fixed and stained with anti–BrdU-FITC antibody and analyzed by flow cytometry to determine the percentage of S phase cells. The average BrdU incorporation percentage is shown for 3 independent K562 vector control or AEtr-expressing pools. Error bars show standard deviation (SD) of 2 different AEtr pools.
