AEtr reduces mitotic arrest in several spindle poisons and in primary cells. (A) K562 cells stably expressing AEtr or vector control cells were treated with 0.1 μg/mL colchicine for 18 hours. PI-stained cells were detected by flow cytometry, and the percentage of mitotic cells is indicated in the bar graph for 2 independent experiments. (B) K562 cells stably expressing AEtr or vector control were treated with 100 nM paclitaxel for 24 and 40 hours. PI-stained cells were detected by flow cytometry, and the percentage of mitotic cells is indicated in the bar graph for 2 independent experiments. (C) Primary bone marrow cells transduced with control EGFP virus or virus encoding AEtr and EGFP were treated with (+) and without (−) 0.1 μg/mL nocodazole for 20 hours. Mitotic index was measured after Hoechst 33342 staining of cells to gate the cells in G2/M phase of the cell cycle. The bar graph shows the flow cytometry data averaged for 3 independent experiments. The asterisk indicates a significant difference between AEtr and control cells after nocodazole treatment. Error bars show SD of the mean from 2 or 3 independent experiments as indicated in each panel.
