Electron microscopy analysis of BzATP-stimulated DCs. Immature DCs in sucrose solution at a concentration of 106/mL at 37°C were left untreated (A-B) or stimulated with 200 μM BzATP (C-D) for 10 minutes. Cells were then detached from flasks, fixed with glutaraldehyde, and processed for electron microscopy. Morphometric analysis indicated a microvesicle size in the range between 100 nm and 1 μm.