IL-29 and IFNα differentially regulate the surface IFNγR1 expression on monocyte-derived macrophages. (A) Monocyte-derived macrophages were treated with IL-29, IL-28A, IL-28B, or IFNα for 5 hours, and then stained with antibodies against IFNγR1. The specificity of the staining was controlled with the appropriate isotype antibodies. (B) Monocyte-derived macrophages were treated with IL-29 or IFNα for 5 hours, and then further stimulated with IFNγ (10 ng/mL) for another 20 hours. Cells were then harvested and stained with antibodies against HLA-DR and HLA-ABC. The specificity of the staining was controlled with the appropriate isotype antibodies.