Figure 5
![Figure 5. FdUMP[10] causes DNA damage and induces apoptosis. (A) Immunofluorescence for γH2AX foci. K562 cells were treated with the indicated drug for 24 hours and then assayed for the presence of γH2AX foci. Secondary Ab was conjugated with Alexa Fluor 594, and images were captured with an Olympus IX70 inverted fluorescent microscope equipped with a Retiga 2000R digital color camera and using an LPlanFl 40×/0.40 objective. Images were analyzed with Image Pro Plus 5.1 software. (B) Flow cytometry of annexin V assay. THP-1 or HL60 cells were treated with the indicated drug for 48 hours and then labeled with annexin V and propidium iodide (PI) and analyzed by flow cytometry. Numbers in bold denote percentage of cells in each quadrant.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/119/15/10.1182_blood-2011-06-362442/5/zh89991288960005.jpeg?Expires=1735823845&Signature=agMUfIHxWgZC0h03EeZrbfgfvoCBqsCkRVUe6t4yZdCSPTbZZdWBH5BNUaf8xgc4wMg90klO-pc9aR47AmOx~r2YEi5YUIQhCqQnLQ1Mus46hAwPZrYr2Ft3S-nhUB4XAxPyiamz-bLg1okEzVXbQSThA8qzlRVYRGFf3ldOG9gKpipqxAcG8-haOeRbXz0O13d~5X8PPCr-IO6cSV1xik-RSL8EIysvRvUtP80Ietla7sXUE38EgIdMerYqzUBSSmhb--DHsBXL~yW-1h8OpOQF98BMR0MT8~wI85Qlam-VZjbR44hW5~d23txWihR~j-T2hPvQuRrV-Sa5oQrGKQ__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
FdUMP[10] causes DNA damage and induces apoptosis. (A) Immunofluorescence for γH2AX foci. K562 cells were treated with the indicated drug for 24 hours and then assayed for the presence of γH2AX foci. Secondary Ab was conjugated with Alexa Fluor 594, and images were captured with an Olympus IX70 inverted fluorescent microscope equipped with a Retiga 2000R digital color camera and using an LPlanFl 40×/0.40 objective. Images were analyzed with Image Pro Plus 5.1 software. (B) Flow cytometry of annexin V assay. THP-1 or HL60 cells were treated with the indicated drug for 48 hours and then labeled with annexin V and propidium iodide (PI) and analyzed by flow cytometry. Numbers in bold denote percentage of cells in each quadrant.
