AURKA_207-215–specificTCR-transduced CD8⁺ T cells display epitope-specific functionality. (A) Representative flow cytometry plots showing staining of AURKA_207-215–specificTCR-transduced CD8⁺ T cells with anti-TCR Vβ12 mAb and HLA-A*0201/AURKA_207-215 tetramer. (B) The same AURKA_207-215TCR-transduced CD8⁺ T cells shown in panel A were tested in ⁵¹Cr-release assays against C1R (negative control) and C1R-A2 cells pulsed with the indicated concentrations of AURKA_207-215 peptide. The parental AUR-2 CTL clone was tested in parallel. E/T indicates effector:target ratio. (C) IFN-γ production by AURKA_207-215–specificTCR-transduced CD8⁺ T cells was measured in a similar format to that described for panel B. (D) Effects of HLA class I and class II blockade on the production of IFN-γ by AURKA_207-215–specificTCR-transduced CD8⁺ T cells stimulated with cognate peptide-loaded (1μM) C1R-A2 cells. (E) Cytotoxic activity of AURKA_207-215–specificTCR-transduced CD8⁺ T cells against unpulsed or cognate peptide-loaded (1μM) C1R-A2 cells as a function of effector:target (E/T) ratio. Error bars represent SDs.