AURKA_207-215–specific TCR-transduced CD8⁺ T cells kill freshly isolated leukemia cells in vitro. Freshly isolated HLA-A*0201⁺ (n = 3) or HLA-A*0201⁻ (n = 3) acute or chronic myeloid leukemia cells overexpressing AURKA mRNA were used as targets in ⁵¹Cr-release assays with AURKA_207-215–specific TCR-transduced CD8⁺ T cells at the indicated effector:target (E/T) ratios. AML, acute myeloid leukemia; BC, blast crisis; CML, chronic myeloid leukemia. M1, M2, and M4 refer to French-American-British classification subtypes (* indicates the expression of AURKA mRNA relative to the mean expression levels across 5 PBMC samples from healthy donors was determined by qRT-PCR and calculated using the comparative ΔCt method). Error bars represent SDs.