AURKA_207-215–TCR transduced CD4⁺ T cells display antigen-specific Th1 cytokine production. (A) A representative flow cytometry plot showing surface Vβ12 expression by AURKA_207-215–specificTCR-transduced CD4⁺ T cells. (B) AURKA_207-215–TCR transduced CD4⁺ T cells produce IFN-γ in response to cognate peptide-loaded (1μM) C1R-A2 cells; unpulsed or irrelevant (HIV p17 Gag SL9) peptide-pulsed C1R-A2 cells were used as negative controls. Cognate antigen-specific IFN-γ production was reduced to background levels in the presence of anti-HLA class I blocking mAb and inhibited in the presence of anti-HLA class II blocking mAb. APC, antigen-presenting cell. Error bars represent SDs.