FOXO1 expression in B-cell subsets and in BCL cell lines. (A) Expression of FOXO1 mRNA in tonsillar CD19⁺ cells, cHL, BL, FL, DLBCL, and pre-BCL cell lines was assessed by quantitative RT-PCR as described in “Quantitative RT-PCR.” Data are mean ± SD of target gene to reference gene (FOXO1/RPL13A) ratio. (B) Expression of FOXO1 mRNA in B-cell subsets. The naive, GC, memory cells (MEM), and CD19⁺ cells were isolated from the fresh surgical material by magnetic cell sorting. The FOXO1 mRNA expression was measured by quantitative RT-PCR, and the data were analyzed by comparative C_t method. The figure represents mean of relative expression ± SD. (C) Expression of FOXO1 protein in human tonsillar CD19⁺ cells and in cHL cell lines. The proteins from samples of CD19⁺ cells and cultured lymphoma cell lines were separated by PAGE, and FOXO1 expression was assessed by immunoblot.