American Society of Hematology

Figure 3

$Figure 3. Bmi-1 induces self-renewal and transforms B-lymphoid progenitors. (A) Frequency of leukemia-initiating cells in Scl/p210;Bmi1 mice in unfractionated and sorted cell fractions. Results are presented as cell input equivalents (P < .05, n = 8-10 mice per group). (B) Representative FACS contour diagram and histogram showing expression (in red, mean fluorescence intensity, MFI) of B220 on EGFP+ cells in Ntg;MOCK, Ntg;Bmi1, Scl/p210;MOCK, and Scl/p210;Bmi1 mice. (C) Relative mRNA expression (quantitative RT-PCR, 2ΔCt) analysis of Scl, Hmgb3, Cbx5, Runx1, and BCR-ABL (b3a2) in EGFP+ B-lymphoid progenitors isolated from Scl/p210;Mock (Mk) and Scl/p210;Bmi1 mice. ND indicates not detected. (D) Relative mRNA expression (quantitative RT-PCR, 2ΔCt) analysis of Scl, and BCR-ABL (b3a2) in EGFP+ HSCs isolated from Scl/p210;Mock (Mk) and Scl/p210;Bmi1 mice. (E) Relative mRNA expression (quantitative RT-PCR, 2ΔCt) analysis of Scl, and BCR-ABL (b3a2) in EGFP+ MPPs isolated from Scl/p210;Mock (Mk) and Scl/p210;Bmi1 mice. (F) Relative mRNA expression (quantitative RT-PCR, normalized) analysis of p16, Ebf1, Pax5, and Ikzf1 performed on EGFP+ B-lymphoid progenitors isolated from primary (first) and tertiary (third) Scl/p210;Bmi1 mice. Data were normalized to the leukemic cells obtained from primary (first) recipient mice.$

Bmi-1 induces self-renewal and transforms B-lymphoid progenitors. (A) Frequency of leukemia-initiating cells in Scl/p210;Bmi1 mice in unfractionated and sorted cell fractions. Results are presented as cell input equivalents (P < .05, n = 8-10 mice per group). (B) Representative FACS contour diagram and histogram showing expression (in red, mean fluorescence intensity, MFI) of B220 on EGFP⁺ cells in Ntg;MOCK, Ntg;Bmi1, Scl/p210;MOCK, and Scl/p210;Bmi1 mice. (C) Relative mRNA expression (quantitative RT-PCR, 2^ΔCt) analysis of Scl, Hmgb3, Cbx5, Runx1, and BCR-ABL (b3a2) in EGFP⁺ B-lymphoid progenitors isolated from Scl/p210;Mock (Mk) and Scl/p210;Bmi1 mice. ND indicates not detected. (D) Relative mRNA expression (quantitative RT-PCR, 2^ΔCt) analysis of Scl, and BCR-ABL (b3a2) in EGFP⁺ HSCs isolated from Scl/p210;Mock (Mk) and Scl/p210;Bmi1 mice. (E) Relative mRNA expression (quantitative RT-PCR, 2^ΔCt) analysis of Scl, and BCR-ABL (b3a2) in EGFP⁺ MPPs isolated from Scl/p210;Mock (Mk) and Scl/p210;Bmi1 mice. (F) Relative mRNA expression (quantitative RT-PCR, normalized) analysis of p16, Ebf1, Pax5, and Ikzf1 performed on EGFP⁺ B-lymphoid progenitors isolated from primary (first) and tertiary (third) Scl/p210;Bmi1 mice. Data were normalized to the leukemic cells obtained from primary (first) recipient mice.

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