Proliferative potential of CFCs in 34DP, 34HC, and total EL cell fractions. 34DP, 34HC, and total EL cells were plated in methylcellulose medium. After 14 days of culture, hematopoietic colonies were scored either as HPP-CFC or as LPP-CFC. (A) HPP-CFC and LPP-CFC number normalized to 1.104 cells for each cell population. (B) Typical HPP-CFUs and LPP-CFUs from each cell population (scale bar, 2 mm). The number of cells seeded in methylcellulose is indicated on the left top of each picture. (C) Cell number fold increase for each cell population. (D) Number of secondary CFCs from primary CFCs for each cell population. Each histogram represents the mean value of triplicates with standard deviation in one representative experiment from 3 independent experiments. ELs (7.0-8.4 weeks old) were used for this study.
