Kinetics of CD34⁺ HPs, CD45^high mature HCs, myeloid, NK, and B-lymphoid cells in long-term culture of human EL 34DP cells compared with 34HCs. 34DP and 34HC EL cells were grown on MS-5 stromal cells. After 14, 25, and 35 days, the whole content of cultures was harvested and stained with antibodies to human lineage-specific markers (A) and human CD34, CD45 (B). (A) Lineage-specific stainings of HCs obtained after 14, 25, and 35 days of culture on MS-5. 34DP cells and 34HCs produce CD33⁺, CD15⁺, CD14⁺ myeloid, CD94⁺, CD161⁺, CD56⁺ NK, and CD19⁺, CD10⁺ B-lymphoid cells. 34DP cells produce the same progeny as 34HCs but with delayed and prolonged kinetics. Data are representative of 3 independent experiments performed on 7.0- to 8.4-week-old EL cells. D, day. (B) CD34 and CD45 stainings of HCs obtained after 14, 25, and 35 days of culture on MS-5. The loss of CD34⁺ progenitors and the production of CD45^high cells are delayed in cultures initiated with 34DP cells compared with cultures initiated with 34HCs. Markers are shown on the x-axis. Numbers indicate percentages of positive cells for each marker. Dotted lines discriminate between CD45^low and CD45^high cells. Data are representative of 3 independent experiments performed on 7.0- to 8.4-week-old EL cells. D indicates day.