Rapid loss of VE-cadherin in EL DP cells after in vitro culture on MS-5 stroma. Flow cytometric analysis of total liver cells triple stained with anti-CD45-fluorescein isothiocyanate, anti-CD34-allophycocyanin, and anti-VE-cadherin-phycoerythrin mAbs shows that as soon as 24 hours of culture on MS-5 stroma, almost no VE-cadherin⁺ cells are detected anymore in the DP VECAD⁺CD45⁺ cell fraction. The black gate delimitates the DP VECAD⁺CD45⁺ cell fraction. Numbers indicate percentages of positive cells in the corresponding quadrants. Data are representative of 3 independent experiments performed on 7.0- to 8.4-week-old EL cells. D indicates day.