Long-term hematopoietic reconstitution of NOD/SCID mice by EL 34DP cells and 34 HCs. (A) Samples of 5000 34DP cells, and 5000, 50 000, and 500 000 34HCs sorted from 7.0- to 8.4-week-old human EL cells were injected into sublethally irradiated NOD/SCID mice, and mice were tested for the presence of human CD45⁺ cells in their BM 4 months after transplantation. Each dot represents the level of reconstitution of individual mouse. Median of reconstitution is reported (black lines). (B) Flow cytometric analysis of NOD/SCID recipient mouse BM 4 months after transplantation of 5000 34DP cells sorted from a 7-week-old human EL. 34DP cells demonstrated multilineage engraftment with an atypical proportion of B-lymphoid and myeloid cells as assessed by triple-staining with human specific mAbs to CD45, CD34, and CD38 and double-staining with human specific mAbs to CD45 and either myeloid (CD33, CD15, CD11b, CD16), B-lymphoid (CD19), or NK (CD94) cells. In addition, a significant fraction of primitive CD34⁺CD38^low and mature CD34⁺CD38⁺ human progenitors were still found in the BM of the injected mouse. Numbers indicate percentages of positive cells in the corresponding quadrants. Data are representative of 3 independent experiments performed on 7.0- to 8.4-week-old EL cells.