Figure 4
Effect of P2Y6 receptor antagonist MRS 2578 on TNF-α–induced gene expression in vascular endothelia. Vascular endothelia (HMEC-1) were pretreated with vehicle (DMSO) or MRS 2578 (10μM) and after 30 minutes were exposed to TNF-α (10 ng/mL). Transcript levels of IL-8 (A), VCAM (B), or intercellular adhesion molecule (C) were determined by real-time RT-PCR relative to housekeeping gene β-actin 2 hours after TNF-α exposure (n = 4).
