Figure 5
Stat5 is not required for transformation mediated by KrasG12D. BM from pI:pC-treated control and MxCre; Stat5fl/fl mice were infected with retroviruses expressing Jak2V617F, BCR-ABL, or KrasG12D. Transduced BM cells (1 × 105) were then plated in methylcellulose medium without any cytokine (Methocult M3234). Cytokine-independent CFU-GM colonies (A) were counted after 7 days and CFU-E colonies (B) were counted after 2 days (n = 4). Asterisks show significant differences by t test (*P < .05; **P < .005). Data are shown as mean ± SEM.
