American Society of Hematology

Figure 1

$Figure 1. Detection of BCR-ABL mRNA transcripts in bone marrow subpopulations of CML-Dx and MMR patients. (A) Sorting strategy of MACS-enriched CD34+ cells. The lineage-negative subfraction was divided into lin−CD34+CD38− cells, enriching for HSCs, and a lin−CD34+CD38+ fraction that was further gated according to IL-3Rα and CD45RA staining into CMP, GMP, and MEP as indicated. (B) Quantitative real-time BCR-ABL PCR, normalized to ABL. Columns represent mean BCR-ABL NCN ± SEM of sorted subfractions (as indicated) from CML patients at diagnosis (CML-Dx) or during MMR (**P < .01; *P < .05 according to 1-way ANOVA; Kruskal-Wallis test with Dunn multiple comparison posttesting).$

Detection of BCR-ABL mRNA transcripts in bone marrow subpopulations of CML-Dx and MMR patients. (A) Sorting strategy of MACS-enriched CD34⁺ cells. The lineage-negative subfraction was divided into lin⁻CD34⁺CD38⁻ cells, enriching for HSCs, and a lin⁻CD34⁺CD38⁺ fraction that was further gated according to IL-3Rα and CD45RA staining into CMP, GMP, and MEP as indicated. (B) Quantitative real-time BCR-ABL PCR, normalized to ABL. Columns represent mean BCR-ABL NCN ± SEM of sorted subfractions (as indicated) from CML patients at diagnosis (CML-Dx) or during MMR (**P < .01; *P < .05 according to 1-way ANOVA; Kruskal-Wallis test with Dunn multiple comparison posttesting).

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