Effects of sustained expression of miR-cluster 99b/let-7e/125a on the stem cell compartment. (A) CAFC data showing the number of HSPCs in the EGFP⁺ BM fraction of 3 individual empty vector and (nonmorbid) miR-cluster 99b/let-7e/125a mice at ∼ 21 weeks posttransplantation. (B) Frequency of LT-HSCs (Lin⁻Sca-1⁺c-Kit⁺CD48⁻CD150⁺) per 1 × 10⁶ EGFP⁺ BM cells at 21 weeks posttransplantation. Analyses were performed using FlowJo software (TreeStar), followed by quantification of the number of LT-HSCs (n = 3/group, same mice as described in panel A). Shown is the mean ± SEM. (C) Representative FACS plots of the EGFP⁺ primitive BM compartment at 21 weeks after primary transplantations. (D) Cell-type distribution in the blood of empty vector and miR-cluster 99b/let-7e/125a mice upon secondary transplantations as assessed by FACS at 24 weeks posttransplantation. Shown is the mean ± SEM (n = 15 mice/group). (E) Chimerism levels at 6 and 24 weeks after secondary transplantation (n = 15 mice/group). GM indicates granulocytes/macrophages; B, B lymphocytes; T, T lymphocytes; and O, other cell types.