GM-CSF regulates DC development through an integrated molecular network. The signaling modules activated by GM-CSF from an integrated network with overlapping and separate functions that enable an adequate response to a wide range of situations. The currently known functions of the specific cascades in the regulation of GM-CSF–induced DC development are shown. Of the signaling proteins contributing to GM-CSF–driven DC development, JAK2-activated STAT5 is the clearest regulator of differentiation. The main role of the PI3K-PKB signaling module is in promoting expansion and survival of DC precursors rather than their differentiation, although its activity is also required to generate DCs with full functionality. Finally, activation of MEK/ERK and canonical NF-κB transcription factors is associated with differentiation and survival.