H3K79 methylation in the Gata2 and Pu1/Sfpi1 loci. ChIP was performed on chromatin from the G1E cell line to assess H3K79 mono-, di-, and tri-methylation of promoter and regulatory sites of Gata2 and Pu1/Sfi1 loci in G1E cells. At the Gata2 locus (A), which is actively transcribed, the 1S and 1G promoters (1S-P, 1G-P) and the first exon following each promoter (1S, 1G), as well as a regulatory element in intron4 (Int4), were examined. At the silenced Pu1/Sfi1 locus (B), we examined 2 upstream regulatory elements (UREs), 5′URE and 3′URE, as well as the promoter region. The data represent the mean of 3 independent experiments.