Cotransfer of the gene-modified CD4 and CD8 T cells shows synergistic effect in an antigen-expressing lymphoma model. E.G7 tumor-bearing B6 mice as described in Figure 4 were either untreated or injected intravenously with EV-transduced or CTC28-transduced OT-I T cells (2 × 10⁶) alone (A) or in combination with EV-transduced or CTC28-transduced OT-II T cells (1 × 10⁶; B). Mean tumor size of at least 5 mice per group was recorded (*P = .0029; Wilcoxon matched pairs test). Results are representative of 2 (A) or 3 independent experiments (B).