Expression profiles of CRLF2 by NCI risk group.CRLF2 expression by NCI risk group is shown along with the corresponding IKZF1 deletions and mutations, CRLF2 lesions and mutations, and JAK mutations. (A-B) All samples for NCI HR and SR, respectively, with the red box indicating the cases with “high” CRLF2 expression. The threshold for high was set at the point where the CRLF2 lesion with the lowest expression (ΔC_t = 8.08) was found. (C-D) Enlarged regions within the boxes for NCI HR and NCI SR, respectively, to help illustrate the patterns of lesions and mutations within each. Black dot represents CRLF2 intensity; yellow circle, IGH@-CRLF2; red square, P2RY8-CRLF2; blue diamond, JAK mutations; yellow triangle, IKZF1 deletions or mutations; and green circle, CRLF2 F232C mutation.