Cocaine-mediated up-regulation of PDGF-BB in HBMECs. (A) Total RNA isolated from HBMECs was subjected to real-time PCR analysis by the use of primer sets specific for PDGF-A, PDGF-B, and PDGF-C. Cocaine dramatically up-regulated PDGF-B chain mRNA expression, with moderate change in PDGF-C and no change in expression of PDGF-A. (B) Dose curve of cocaine-mediated induction of PDGF-B RNA by real-time reverse transcription PCR in HBMECs. (C) Whole cell lysates from cocaine exposed cells were subjected to Western blot analysis with the use of antibodies specific for PDGF-BB. Cocaine induced time-dependent induction of PDGF-BB protein in HBMECs. All data are presented from 3 separate experiments.