Classic aggregometry and flow chamber perfusion assay. (A) Scheme representing the integrin and nonintegrin receptors studied, with their natural ligand, agonist, and antagonist indicated. (B) Aggregation of control, Glanzmann, and LAD-III platelets (left) or control platelets preincubated with different antagonists (right) upon stimulation with 10 μg/mL collagen measured by light transmission aggregometry. (C) Binding of control, Glanzmann, and LAD-III platelets (left) or control platelets preincubated with different antagonists (right) to collagen-coated slides. Pictures were taken using an EVOS fl (fluorescence) digital inverted microscope by Advanced Microscopy Group at 600× magnification using an 60×/1.35 oil objective. The imaging medium was PBS. The EVOS fl contains a CCD camera and EVOS fl software was used for acquiring images. Adobe Suite CS 5 Photoshop was used to adjust brightness and contrast.