Murine model of bcCML. (A) Murine stem cell virus construct containing BCR/ABL and GFP. (B) MSCV construct containing Nup98/HoxA9 and YFP. (C) Schematic representation of the transplant strategy used to produce the leukemic mice used. (D) Flow cytometric gating strategy to identify leukemic cells as GFP⁺ and YFP⁺. (E) Representative anti-GFP immunohistochemistry of the femur's marrow space at the metaphysis. GFP is visualized by brown staining, with a hematoxylin counterstain. Top panel: 20× objective; bottom panel: 60× objective. (F) Flow cytometric data represent bcCML cells as a percentage of total marrow mononuclear cells. (G) Flow cytometric data representing bcCML cells as a percentage of total spleen. (H) Total peripheral blood mononuclear cells over the course of 10 days. *P ≤ .05. **P ≤ .01. ***P ≤ .001. n = 5 mice per time point. Bar represents SEM in this and subsequent experiments.